Includes bibliographical references and index.

Introduction to optical tweezers : background, system designs, and commercial solutions / Joost van Mameren, Gijs J.L. Wuite, and Iddo Heller -- Optical trapping and unfolding of RNA / Katherine H. White and Koen Visscher -- DNA unzipping and force measurements with a dual optical trap / Ismaı̈l Cissé, Pierre Mangeol, and Ulrich Bockelmann -- Probing the force generation and stepping behavior of cytoplasmic dynein / Arne Gennerich and Samara L. Reck-Peterson -- Brief introduction to single-molecule fluorescence methods / Siet M.J.L. van den Wildenberg, Bram Prevo, and Erwin J.G. Peterman -- Fluorescent labeling of proteins / Mauro Modesti -- Fluorescence imaging of single kinesin motors on immobilized microtubules / Till Korten ... [et al.] -- Exploring protein superstructures and dynamics in live bacterial cells using single-molecule and superresolution imaging / Julie S. Biteen, Lucy Shapiro, and W.E. Moerner -- Fluorescence microscopy of nanochannel-confined DNA / Fredrik Persson, Fredrik Westerlund, and Jonas O. Tegenfeldt -- Fluorescence correlation spectroscopy / Patrick Ferrand, Jérôme Wenger, and Hervé Rigneault -- Introduction to atomic force microscopy / Pedro J. de Pablo -- Sample preparation for SFM imaging of DNA, proteins, and DNA-protein complexes / Dejan Ristic, Humberto Sanchez, and Claire Wyman -- Single-molecule protein unfolding and refolding using atomic force microscopy / Thomas Bornschlögl and Matthias Rief -- How to perform a nanoindentation experiment on a virus / Wouter H. Roos -- Magnetic tweezers for single-molecule manipulation / Yeonee Seol and Keir C. Neuman -- Probing DNA topology using tethered particle motion / David Dunlap ... [et al.].